KMID : 0613820140240020105
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Journal of Life Science 2014 Volume.24 No. 2 p.105 ~ p.111
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Identification of Equine Heat Shock Proteins Gene and Their mRNA Expression Analysis after Exercise
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Cho Hyun-Woo
Park Jeong-Woong Choi Jae-Young Sivakumar S. Kim Nam-Young Shin Teak-Soon Cho Seong-Keun Kim Byeong-Woo Cho Byung-Wook
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Abstract
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The purpose of this study was to characterize equine heat-shock protein (Hsp) genes and analyze their expression pattern in various horse tissues and blood leukocytes after exercise. In a previous study, RNA sequencing of blood and skeletal muscles of thoroughbreds before and after exercise was performed using differently expressed gene (DEG) analysis. Three Hsp genes (HspH1, Hsp90${\alpha}$ ¼ö½Ä À̹ÌÁö and Hsp70) were selected by DEG analysis and were found to be differentially expressed in either blood or muscle. To validate and extend previous observations on these genes, we performed RT-PCR analyses of horse tissue as well as real-time qPCR analyses of blood leukocytes after exercise. mRNA expression of these Hsp genes was found to be ubiquitous in the analyzed tissues (including thyroid, colon, skeletal muscle, cecum, kidney, spinal cord, heart, and lung). In addition, Hsp mRNA expression of these genes in extracted whole blood increased after 120 minutes of exercise compared to the baseline condition. These results are in agreement with the results of human and other experimental animals, suggesting that regulatory mechanisms that are responsible for upregulation of Hsp gene transcription may be conserved among species. Further investigations to correlate Hsp gene expression patterns with athletic performance or recovery processes after exercise are warranted.
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KEYWORD
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Equus caballus, exercise, heat shock protein, real-time quantitative PCR, thoroughbred
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